Composite

Part:BBa_K823052

Designed by: Jara Radeck   Group: iGEM12_LMU-Munich   (2012-09-10)
Revision as of 19:12, 23 September 2012 by Jara (Talk | contribs)

lacZ, B. subtilis optimized + RBS + Term

lacZ in Freiburg standard, Bacillus subtilis codon optimized with ribosome binding site and terminator (B0014).

lacZ is a β-galactosidase which cleaves the substrates X-Gal (5-bromo-4-chloro-indolyl-β-D-galactopyranoside) and ONPG (ortho-Nitrophenyl-β-galactoside).

This BioBrick was used for promoter evaluation in B. subtilis.

prefix: GAATTCCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGC

suffix: ACCGGTTAATACTAGTAGCGGCCGCTGCAGT



We used this lacZ gene to evaluate our promoters.

lacZ under the control of Pspac in pSB1C3. Plate was induced with xylose.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None