Coding

Part:BBa_K785004:Design

Designed by: Zining Hou   Group: iGEM12_Fudan_Lux   (2012-09-23)
Revision as of 18:07, 23 September 2012 by Registry (Talk | contribs)

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Lov-HTH (light sensor with Ptet repression) light->PtetO repressed


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 633
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 187
    Illegal AgeI site found at 297
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The HTH motif swap is the main issue in our design. We carefully choose the HTH sequence and swap it into the original amino acid sequence.


Source

The sequence is reverse translated from the protein EL222 http://www.rcsb.org/pdb/explore/explore.do?structureId=3P7N which is from Erythrobacter litoralis. The DNA is synthesized by Invitrogen company.

References