Regulatory

Part:BBa_K875001:Experience

Designed by: Federico Colombo, Elisa Clagnan   Group: iGEM12_Trieste   (2012-09-23)
Revision as of 13:41, 23 September 2012 by Giulio (Talk | contribs) (→‎Applications of BBa_K875001)

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Trieste Team 2012

In liquid assay:

We inoculated a 20ml culture. After overnight growth, we diluted the culture to OD600 = 0.2. Then we aliquoted 200 μl in 8 replicates in a microtiter plate at different concentrations of cumate. The reading was performed in a monochromator at 485-510nm.

"Graph 1"

In the plate assay:


We streaked the culture on LB Agar plates containing different p-cumate concentrations.

"pic 1"

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