Generator

Part:BBa_K743015:Design

Designed by: Bernardo Pollak   Group: iGEM12_UC_Chile   (2012-09-22)
Revision as of 21:14, 22 September 2012 by Juan Alamos (Talk | contribs) (→‎Source)

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after dusk -> LuxAB luciferase (Vibrio fischerii)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 693
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 521
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2080


Design Notes

This part was made by Gibson assembly, therefore it has no scars


Source

Pta promoter was amplified from genomic Synechocystis DNA, you can find references and more information at the biobrick version of this promoter. LuxAB genes were obtained from the Luxbrick

References