Protein_Domain
CBDcex

Part:BBa_K863101:Design

Designed by: Moritz Müller   Group: iGEM12_Bielefeld-Germany   (2012-09-18)
Revision as of 19:45, 22 September 2012 by Mo (Talk | contribs) (→‎Design Notes)

Cellulose binding Domain of Cellulomonas Fimi Exoglucanse (Freiburg-Standard)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 337
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence starts with the rest of the Freiburg-prefix (ATG and NgoMIV-restriction-site)and the A from the startcodon ATG is the last base of the XbaI restriction-site of the Standard 25 Prefix (Read here). I conserved 12 Bases (4 AS) upstream of the cellulose binding domain and 9 bases downstream of the exoglucanase gene and added a short N-terminal (Glycine, Serine) linker. The sequence ends with the AgeI-restriction-site of the Freiburg-Suffix, to make in frame assembly possible.

Source

Cloning-Vector with a CBDcex-Barnase fusion-protein

References