Coding

Part:BBa_K733005:Design

Designed by: Chris Yu Lai Cheong; LI Yiming   Group: iGEM12_HKUST-Hong_Kong   (2012-09-16)
Revision as of 18:11, 22 September 2012 by Wyq.hkust (Talk | contribs) (→‎Source)

ybdN+Bmp2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The mouse BMP-2 DNA sequence has an EcoRI cutting site and this site is within the codon of the DNA sequence. To standardize the biobrick, we try to do a point mutation on mouse BMP-2 after we amplify it from PCR. The purpose is to remove the EcoRI cutting site and remain the correct codon for protein translation of BMP-2.

Source

Extract BMP-2 from mouse genomic DNA by PCR. Extract signal peptide YbdN from Bacillus subtilis genomic DNA by PCR. Ligation of YbdN and BMP2 achieved by overlapping PCR.

References