Composite
Part:BBa_K733010
Designed by: WANG, Yuqi Group: iGEM12_HKUST-Hong_Kong (2012-09-16)
Ptms+RBS+ydcD.
This construct contains a low efficient constitutive promoter pTms with an antitoxin gene ydcD (endB). The gene ydcD can express a protein named YdcD, which can counteract with a protein EndoA expressed by a toxin gene ydcE (ndoA). (Pellegrini et al., 2005) Besides, the low efficiency promoter will contribute to the low level expression of YdcD.
pTms promoter: BBa_K733001
ydcD: BBa_K733003
ydcE: BBa_K733004
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Reference
Pellegrini O, Mathy N, Gogos A, Shapiro L, and Condon C. "The Bacillus subtilis ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor.." Molecular microbiology. 56.5 (2005): 1139-1148. Print.
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Categories
Parameters
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