Composite

Part:BBa_K743013

Designed by: Bryon Silva   Group: iGEM12_UC_Chile   (2012-09-22)
Revision as of 16:33, 22 September 2012 by Juan Alamos (Talk | contribs)

Arabinose --> HIV protease secretion

OmpA - HIV autocleavage secretion system

Upon addition of arabinose to the medium, the inducible Pbad promoter drives the expression of outer membrane protein A fused to a extracellular linker (ompA brick) followed by a cleavage site recognizable by HIV protease wich is linked to that protease coding sequence.

We expect for the protease to recognize it´s own cleavage site and to be released to the medium.

It was used by [http://2012.igem.org/Team:UC_Chile 2012 UC Chile team] to secrete GFP. You can check the results HERE LINK!


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


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