Part:BBa_K743010

psb1C3_IntS recombination plasmid with RFP

psb1C3_IntS recombination plasmid is designed to integrate any desired biobricks or other sequences into Synechocystis PCC6803 chromosome and/or pSYSX endogenous plasmid while interrupting CopS gene coding sequence.

Synechocystis naturally undergoes double homologous recombination so the use of recombination sites flanking prefix and suffix were designed with complete homology to CopS gene, present in the chromosome and in a endogenous plasmid. As CopS is essential for copper stress tolerance in this bacteria, by using this plasmid a succeptible strain incapable of thriving in the enviroment is created.(see references for more information)

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 1135
  Illegal suffix found in sequence at 2226
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1135
  Illegal SpeI site found at 2227
  Illegal PstI site found at 2241
  Illegal NotI site found at 1141
  Illegal NotI site found at 2234
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1135
  Illegal XhoI site found at 3689
  Illegal XhoI site found at 4581
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 1135
  Illegal suffix found in sequence at 2227
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 1135
  Illegal XbaI site found at 1150
  Illegal SpeI site found at 2227
  Illegal PstI site found at 2241
  Illegal NgoMIV site found at 867
  Illegal AgeI site found at 1937
  Illegal AgeI site found at 2049
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2783