Coding

Part:BBa_K896007

Designed by: Shih-Hao Chen   Group: iGEM12_NYMU-Taipei   (2012-09-22)
Revision as of 15:05, 22 September 2012 by Whimsical (Talk | contribs)

Nap( Nitrate->Nitrite reductase)

A type of respiratory NO3-reductase is a water-soluble, periplasmicallylocated protein known as Nap. We acquire the Nap gene from Desulfovibrio desulfuricans ATCC 27774, the function of Nap is to transfer NO into nitrogen.

Nap cloning.jpg

The Prokaryotic Nitrate Reductases David J. Richardson, Rob J.M. van Spanning and Stuart J. Ferguson

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1594
    Illegal PstI site found at 2427
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1594
    Illegal PstI site found at 2427
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1594
    Illegal BamHI site found at 1803
    Illegal XhoI site found at 2361
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1594
    Illegal PstI site found at 2427
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1594
    Illegal PstI site found at 2427
    Illegal NgoMIV site found at 2983
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1342
    Illegal SapI.rc site found at 1903


[edit]
Categories
Parameters
None