Composite
psb1C3_int

Part:BBa_K743009:Design

Designed by:   Group: iGEM12_UC_Chile   (2012-09-22)
Revision as of 14:46, 22 September 2012 by Juan Alamos (Talk | contribs) (→‎Design Notes)

psb1C3_IntKR recombination plasmid with RFP under psaAB promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2657
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2657
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2657
    Illegal XhoI site found at 3154
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2657
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2657
    Illegal AgeI site found at 1270
    Illegal AgeI site found at 1382
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 511


Design Notes

This composite part was assembled by Gibson assembly technique. It is designed to be edited by Gibson assembly, and not by standard biobrick assembly, because of a EcoR1 restriction site present in [BBa_K743001 RS2 recombination sequence]

Source

Go to https://parts.igem.org/Part:BBa_K743008 for information about the plasmid backbone. Both the psaAB promoter and the RFP coding sequence were obtained from the registry.

References