Regulatory
PfadR

Part:BBa_K861060

Designed by: Kuanwei Sheng   Group: iGEM12_WHU-China   (2012-09-08)
Revision as of 14:18, 22 September 2012 by Tinaa (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

PfadR, synthetic promoter with tandem FadR binding site

At least to our knowledge, there is no promoter exists in nature that can respond solely to FadR since those promoters are often regulated by glucose concentration or oxidative stress and many other factors. We decided to design an artificial promoter to fulfill this task. Specifically, a tandem FadR binding site in FadL promoter is placed downstream of promoter BBa_J23110 with a 3bp-overlap. We tested its function in M9 medium with oleic acid as sole carbon source in plasmid pSB6A1.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aPfadR, synthetic promoter with tandem FadR binding site