Part:BBa_K896006:Design

NirN-NirS (Nitrite->Nitric oxide reductase)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1703
  Illegal BamHI site found at 1483
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 52
  Illegal NgoMIV site found at 168
  Illegal NgoMIV site found at 220
  Illegal NgoMIV site found at 604
  Illegal NgoMIV site found at 1297
  Illegal NgoMIV site found at 2305
  Illegal AgeI site found at 3169
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2347
  Illegal BsaI.rc site found at 2479
  

Design Notes

Cloning of NirN-NirS gene:

SQR gene came from Synechococcus elongatus PCC7002 because the hypersaline strain S. elongatus PCC 7002, which is already sequenced, is 96% similar to O.limnetica SQR gene.

Source

YES