Regulatory
Pcar

Part:BBa_K861171

Designed by: Kuanwei Sheng   Group: iGEM12_WHU-China   (2012-09-07)
Revision as of 10:23, 22 September 2012 by Shinkwee (Talk | contribs)

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Pcar, synthetic promoter repressed by CRP

This is a promoter designed for the Eschaerichia coli. It includes the consensus CRP-binding site and the altered consensus RNA polymerase-binding site which have a overlap of several base pairs. Due to the steric hindrance between CRP and RNA polymerase, gene downstream of the promoter will be repressed.In the cells, low glucose concentration will result in increased activity by adenylate cyclase, rising up the concentration of cAMP in cell. cAMP binds to the CRP, which, in its bound form, is able to bind tightly to the specific DNA site in the promoter and repress the gene downstream. On the contrary, high glucose concentration will result in the expression of downstream genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
n/aPcar, synthetic promoter repressed by CRP