Device
FlipFlop

Part:BBa_K907003

Designed by: Dong-hui Choe, Soo-in Lee   Group: iGEM12_KAIST_Korea   (2012-09-20)
Revision as of 10:10, 22 September 2012 by Robinald (Talk | contribs)

Binary signal generator, promoter reversed, RBS(reverse) - attB - Promoter(reverse) - attP - RBS

<Part Description>

KAIST BBa K907002.png


This part is composed of 3 elements.
1. RBSs: BBa_B0034, upsteam one is reversed
2. Promoter: BBa_J23119, Bacterial constitutive promoter reversed
3. att sites: Recognition site for Mycobacteriophage Bxb1 integrase/excisionase
We designed this part to generate two different proteins one at a time.

<Application>

KAIST Dual Phase protein generator default.png

KAIST Dual Phase protein generator inverted.png


At the first state, this device promotes the transcription and translation of upstream reversed gene because of its promoter orientation. When Mycobacteriophage Bxb1 integrase recognizes and inverts the sequence between with attB and attP sequence, promoter orientation changed. Then this device promotes the transcription and translation of downstream gene.

This device is tested by GFP and mRFP attached construct. See BBa_K0907004 and BBa_K0907005

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 69
    Illegal NheI site found at 92
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 157
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 22
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 42
    Illegal BsaI.rc site found at 125


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Parameters
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