Generator

Part:BBa_K861051

Designed by: Kuanwei Sheng   Group: iGEM12_WHU-China   (2012-09-06)
Revision as of 08:49, 22 September 2012 by Tinaa (Talk | contribs)

FadR with a RBS and a terminator


FadR is a transcription regulator, which, when not binds to acyl-CoA can either serve as an activator for fatty acid synthetase gene like FabA, FabB and etc. or a repressor for fatty acid degradation gene like FadA, FadB, FadD and etc. After long chain fatty acid is converted to fatty acyl- CoA by FadD, it can bind to FadR. This binding will alter the conformation of FadR, making it unable to bind to the DNA sequence it recognizes. To our knowledge, there is no promoter exists in nature that can respond solely to FadR since those promoters are often regulated by glucose concentration or oxidative stress and many other factors.

This device contains a RBS and a terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None