Generator

Part:BBa_K861003

Designed by: Kuanwei Sheng   Group: iGEM12_WHU-China   (2012-09-21)
Revision as of 06:53, 22 September 2012 by Tinaa (Talk | contribs)

PfadR regulated FadL

Long-chain fatty acids are transported across the cell membrane via a transportation/acyl-activation mechanism involving an outer membrane protein, FadL(BBa_K861500). The fadL gene codes for a 43,000-dalton membrane protein (FLP) which has been implicated as being essential for LCFA transportation. When expressed, FadL can transport the long-chain fatty acids into the bacterium. We assembled FadL with PfadR (BBa_K861060) hoping it will only be overexpressed when fatty acids concentration is high.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1207
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aPfadR regulated FadL