Intermediate

Part:BBa_K861346

Designed by: Kuanwei Sheng   Group: iGEM12_WHU-China   (2012-09-21)
Revision as of 18:17, 21 September 2012 by Shinkwee (Talk | contribs)

IPTG induced promoter with FadA, intermediate part for BBa_K861036

To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins. We put the enzymes in different combination with substrates needed for fatty acid oxidation in a test tube to look for the combination that has the best degradation capability. This is a intermediate part for BBa_K861036.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 168
    Illegal BglII site found at 1044
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 658
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None