Generator

Part:BBa_K861046

Designed by: Kuanwei Sheng   Group: iGEM12_WHU-China   (2012-09-06)
Revision as of 16:47, 21 September 2012 by Shinkwee (Talk | contribs)

PfadR regulated FadA and FadB

In the process of LCFA degradation,the cycles of hydration, oxidation, and thiolytic cleavage are carried out by tetrameric complex consisting of two FadA and two FadB proteins. We assebled FadA and FadB with PfadR (BBa_K861060) hoping they will only be overexpressed when fatty acids concentration is high.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 188
    Illegal BglII site found at 1064
    Illegal BglII site found at 1447
    Illegal BglII site found at 1594
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 678
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None