Device
Part:BBa_K786002:Design
Designed by: Leung Wai Tak Group: iGEM12_Hong_Kong-CUHK (2012-09-21)
Sensory rhodopsin II (SRII) with HtrII & Tar, sensitive to blue-green light
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 37
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 37 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 785
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 37
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 37
Illegal NgoMIV site found at 140
Illegal NgoMIV site found at 398
Illegal AgeI site found at 1703 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1139
Illegal SapI site found at 913
Design Notes
Pfam (version 26.0) was used to predict the domain of fusion protein. Linker was refereed and modified from previous research study of SRII fusion with HtrII.
Source
From genomic sequence of bacterial N. pharaonis (DSM 2160) and E.coli K12.