Not Released
Sample In stock
Experience: Issues
Not Used
Get This Part
Generator

Part:BBa_K914008:Design

Designed by: Denis Samuylov   Group: iGEM12_Paris_Bettencourt   (2012-09-20)
Revision as of 13:24, 21 September 2012 by Denis.Samuylov (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Meganuclease I-SceI controlled by pRha


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The system is leaky and meganuclease cut RS with high efficiency even without pRha induction.

Sequence

The sequence available here was automatically generated by the Parts Registry as a composite part assembled from several basic parts. In fact, during the ligation, one of the created scars is slightly different: the actual sequence of the scar between the RBS and the I-SceI gene is TACTAGAG (and not TACTAG)

Source

Promoter pRha was amplified from the plasmid pJOE3075 (Dr. Altenbuchner). RBS was taken from an iGEM distribution plate (BBa_B0032). Meganuclease was amplified from the chromosome of SMR6316 E.Coli strain (Dr. Rosenberg).

References