Generator

Part:BBa_K907004

Designed by: Dong-hui Choe, Soo-in Lee   Group: iGEM12_KAIST_Korea   (2012-09-20)
Revision as of 13:15, 21 September 2012 by Robinald (Talk | contribs)

Dual phase protein generator(GFP default). mRFP and GFP

Dual phase protein generator(GFP default). mRFP and GFP

This part is derivative of BBa_K907002.

BBa_E1010(mRFP, reversed) and BBa_E0040(wtGFP) are attached both end of BBa_K907002 with overlapping extension PCR. This part generates GFP in E.coli(strain MG1655) normally. When BBa_K907000(Bxb1 integrase) inverts the promoter orientation, it starts to generate mRFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 756
    Illegal NheI site found at 779
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 838
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 703
    Illegal AgeI site found at 10
    Illegal AgeI site found at 122
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 723
    Illegal BsaI.rc site found at 806
    Illegal BsaI.rc site found at 1499


[edit]
Categories
Parameters
emission511nm(maximum, BBa_E0040), 607nm(maximum, BBa_E1010), 536nm(for detection, BBa_E0040), 619nm(for detection, BBa_E1010)
excitation501nm(BBa_E0040), 584nm(BBa_E1010)