Signalling
Part:BBa_K750102
Designed by: Sifan Wang,Yizhen Yan,Jianzhao Chi,Qingshu Wu Group: iGEM12_XMU-China (2012-09-21)
PtetcIT:atc sensor(J23119 promoter) with cI system
This part contains a constitutive promoter(BBa_J23119) and a tetR QPI(BBa_Q0040). It takes (anhydro)tetracyclin as the input signal, and the parts behind will produce corresponding output signal. In our project, this part is ligated with the CI protein expression system() to make up a atc sensor with CI.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
control | R0040 |
device_type | signalling |
n/a | PtetcIT:atc sensor(J23119 promoter) with cI system |
protein | CI |