Coding

Part:BBa_K801092:Design

Designed by: Ingmar Polte   Group: iGEM12_TU_Munich   (2012-09-21)
Revision as of 11:59, 21 September 2012 by Registry (Talk | contribs)

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4-coumarate - coenzym A ligase (4CL) + yeast consensus sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1092
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1659
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1291


Design Notes

-


Source

Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA

http://www.ncbi.nlm.nih.gov/nucleotide/609339/

PMID: 15932991

References