Coding

Part:BBa_K801091:Design

Designed by: Ingmar Polte   Group: iGEM12_TU_Munich   (2012-09-21)
Revision as of 11:52, 21 September 2012 by Registry (Talk | contribs)

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phenylalanine ammonia lyase (PAL) coding region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2090
    Illegal BamHI site found at 352
    Illegal XhoI site found at 403
    Illegal XhoI site found at 466
    Illegal XhoI site found at 484
    Illegal XhoI site found at 562
    Illegal XhoI site found at 763
    Illegal XhoI site found at 1006
    Illegal XhoI site found at 1753
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 922
    Illegal NgoMIV site found at 1168
    Illegal NgoMIV site found at 1336
    Illegal NgoMIV site found at 1479
    Illegal NgoMIV site found at 1813
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 463
    Illegal BsaI site found at 793
    Illegal BsaI site found at 799
    Illegal BsaI.rc site found at 1924
    Illegal SapI site found at 753


Design Notes

-


Source

Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA

http://www.ncbi.nlm.nih.gov/nuccore/AX366866

PMID: 15932991

References