Device

Part:BBa_K786001

Designed by: Leung Wai Tak   Group: iGEM12_Hong_Kong-CUHK   (2012-09-21)
Revision as of 11:50, 21 September 2012 by Registry (Talk | contribs)

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Sensory rhodopsin II (SRII) with HtrII & Tsr, sensitive to blue-green light

Sensory rhodopsin II fusion with HtrII & Tsr, which is sensitive to blue-green light, with sensing spectra covering from 400-500 nm. We used it for sensing blur-green light to achieve phototaxis and switch for downstream gene expression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 37
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 37
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 785
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 37
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 37
    Illegal NgoMIV site found at 140
    Illegal NgoMIV site found at 398
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1139
    Illegal SapI site found at 913
    Illegal SapI.rc site found at 1919


[edit]
Categories
//cds/membrane/receptor
//chassis/prokaryote/ecoli
//classic/signalling/sender
//function/sensor/light
//plasmid/expression
Parameters
device_type
function
protein