Device
Part:BBa_K786001
Designed by: Leung Wai Tak Group: iGEM12_Hong_Kong-CUHK (2012-09-21)
Sensory rhodopsin II (SRII) with HtrII & Tsr, sensitive to blue-green light
Sensory rhodopsin II fusion with HtrII & Tsr, which is sensitive to blue-green light, with sensing spectra covering from 400-500 nm. We used it for sensing blur-green light to achieve phototaxis and switch for downstream gene expression.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 37
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 37 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 785
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 37
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 37
Illegal NgoMIV site found at 140
Illegal NgoMIV site found at 398 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1139
Illegal SapI site found at 913
Illegal SapI.rc site found at 1919
[edit]
Categories
Parameters
//cds/membrane/receptor
//chassis/prokaryote/ecoli
//classic/signalling/sender
//function/sensor/light
//plasmid/expression
//chassis/prokaryote/ecoli
//classic/signalling/sender
//function/sensor/light
//plasmid/expression
device_type | |
function | |
protein |