Composite

Part:BBa_K777001

Designed by: Bianca Genenncher   Group: iGEM12_Goettingen   (2012-09-21)
Revision as of 09:45, 21 September 2012 by B.gene (Talk | contribs)

Tar receptor under the control of constitutive promoter J23100

Fig. 1: Overview of BioBricks containing the Tar gene downstream of Anderson promoters. (Activity according to Berkeley 2006)

The transmembrane chemoreceptor Tar of E. coli mediates chemotaxis towards aspartate and exists as a functional homodimer.

Here we used a set of 8 constitutive Anderson promoters from the 2006 Berkeley group to test how different levels of constitutive Tar expression affect the chemotaxis of E. coli.












Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1323
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 152


[edit]
Categories
//chassis/prokaryote/ecoli
//promoter/anderson
//regulation/constitutive
Parameters
biologyEscherichia coli
chassisE. coli
ligandsAspartate
positive_regulatorsJ23100 constitutive promoter
proteinTar