Plasmid_Backbone

Part:BBa_K801002:Design

Designed by: Georg Schtzinger   Group: iGEM12_TU_Munich   (2012-09-21)
Revision as of 09:31, 21 September 2012 by Giergl (Talk | contribs) (→‎Source)

pTUM102 yeast shuttle vector with pTEF2 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 36
    Illegal suffix found at 705
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 36
    Illegal NheI site found at 3970
    Illegal SpeI site found at 706
    Illegal PstI site found at 720
    Illegal NotI site found at 42
    Illegal NotI site found at 713
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 36
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 36
    Illegal suffix found at 706
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 36
    Plasmid lacks a suffix.
    Illegal XbaI site found at 51
    Illegal SpeI site found at 706
    Illegal PstI site found at 720
    Illegal NgoMIV site found at 194
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2127
    Illegal BsaI.rc site found at 3073
    Illegal SapI site found at 1044
    Illegal SapI site found at 3225


Design Notes

All forbidden restriction sites have been removed from the plasmid backbone, making it RFC25 compatible.


Source

Based on invitrogen's pYES2 plasmid. For more information see the part information of pTUM100 (BBa_K801000).

References