Composite
psb1C3_int

Part:BBa_K743008:Design

Designed by: Bernardo Pollak, Isaac Nuez   Group: iGEM12_UC_Chile   (2012-09-20)
Revision as of 01:42, 21 September 2012 by Juan Alamos (Talk | contribs) (→‎Source)

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psb1C3_IntKR recombination plasmid for Synechocystis PCC6803


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1758
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1758
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1758
    Illegal XhoI site found at 2255
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1758
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1758
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 511


Design Notes

Using a double terminator followed by a reversed antibiotic resistance cds allows the use of the same terminator sequence for both the resistance casette and any desired coding sequence placed upstream


Source

Both BBa_K743001 and BBa_K743000 were PCR amplified from genomic dna. BBa_K743001 and BBa_B0015 were amplified from biobricks.

Please note that BBa_K743001 has a EcoR1 restriction site at position 132, because of this this construct can not be handled as an upstream part. This also should be considered when making analytic digestions.

References