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Part:BBa_K914008:Design

Designed by: Denis Samuylov   Group: iGEM12_Paris_Bettencourt   (2012-09-20)
Revision as of 23:26, 20 September 2012 by Registry (Talk | contribs)

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Meganuclease I-SceI controlled by pRha


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The system is leaky and meganuclease cut RS with high efficiency even without pRha induction.


Source

Promoter pRha was amplified from the plasmid pJOE3075 (Dr. Altenbuchner). RBS was taken from an iGEM distribution plate (BBa_B0032). Meganuclease was amplified from the chromosome of SMR6316 E.Coli strain (Dr. Rosenberg).

References