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Part:BBa_K914005:Design

Designed by: Denis Samuylov   Group: iGEM12_Paris_Bettencourt   (2012-09-20)
Revision as of 23:00, 20 September 2012 by Registry (Talk | contribs)

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Meganuclease I-SceI controlled by pLac


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The pLac promoter is leaky.


Source

Promoter pLac and RBS was taken from an iGEM distribution plate (BBa_R0011 and BBa_B0032). Meganuclease was amplified from the chromosome of SMR6316 E.Coli strain (Dr. Rosenberg).

References