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psb1C3_Int

Part:BBa_K743005:Design

Designed by:   Group: iGEM12_UC_Chile   (2012-09-20)
Revision as of 22:14, 20 September 2012 by Registry (Talk | contribs)

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psb1C3_IntK plasmid with RFP under psbA2 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2684
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2684
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2684
    Illegal XhoI site found at 3181
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2684
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2684
    Illegal AgeI site found at 1178
    Illegal AgeI site found at 1290
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 511


Design Notes

Source

RS1 and RS2 were obtained from Synechocystis genomic DNA, both the kanamycin resistance and the double terminator are from the registry of standard biological parts.

References