DNA
RS1

Part:BBa_K743000:Design

Designed by:   Group: iGEM12_UC_Chile   (2012-09-20)
Revision as of 22:05, 20 September 2012 by Juan Alamos (Talk | contribs) (→‎References)

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Synechocystis PCC6803 neutral recombination site.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 511


Design Notes

According to literature, in Synechocystis the recombination sites must be at least 450 bp long to allow a rasonably high transformation efficiency, being approximately 1200 bp the optimum size.

Source

PCR amplified from synechocystis PCC6803 chromosome, orf slr0370.

References

http://genome.kazusa.or.jp/cyanobase/Synechocystis

Kufryk, G. I., Sachet, M., Schmetterer, G., & Vermaas, W. F. J. (2002). Transformation of the cyanobacterium Synechocystis sp. PCC 6803 as a tool for genetic mapping: optimization of efficiency. FEMS microbiology letters, 206(2), 215-9.

Xu, Y., Alvey, R. M., Byrne, P. O., Graham, J. E., Shen, G., & Bryant, D. A. (2011). Photosynthesis Research Protocols. (R. Carpentier, Ed.)Methods, 684, 273-293.

Kucho, K.-ichi, Aoki, K., Itoh, S., & Ishiura, M. (2005). Improvement of the bioluminescence reporter system for real-time monitoring of circadian rhythms in the cyanobacterium Synechocystis sp. strain PCC 6803. Genes & genetic systems, 80(1), 19-23.