Plasmid
Part:BBa_K802003
Designed by: Sandu Ioana Group: iGEM12_Lyon-INSA (2012-09-20)
Shuttle vector for E. coli and B. subtilis
The shuttle vector is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a low plasmid copy number.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6506
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 6506
Illegal suffix found in sequence at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 6506
Illegal XbaI site found at 6521
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3203
Illegal BsaI.rc site found at 5190
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Categories
Parameters
None |