Part:BBa_K812300
Biobricked pCS2+ plasmid with HSP70 promoter for frogs and chicken
This plasmid is a biobricked version of the plasmid pSC2+(K812000)with HSP70 promoter. This plasmid is made of a bacterial ampicilin resistant backbone. The Biobrick site is surrounded by a 5'UTR expression enhancer, and a poly-A tail with a SV40 3' UTR that enhance the stabilisation of the mRNA and the expression of the protein.
To use this plasmid, you simply have to insert your protein between the EcoRI and PstI site. It is better to add a kozac sequence in front of your gene to enhance its expression. This plasmid contains the standard registry insert J04450 as a negative cloning control.
The Heat Shock Promoter 70 induce the expression of the protein which is after HSP70. The induction can occur heating tadpole from 21°C to 34°C.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3570
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 248
Illegal NotI site found at 3576 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3570
Illegal BglII site found at 3407
Illegal BamHI site found at 3555
Illegal XhoI site found at 3126 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 3570
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 3570
Plasmid lacks a suffix.
Illegal XbaI site found at 3585
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 2611 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 1634
Illegal SapI site found at 551
Illegal SapI.rc site found at 3419
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