Translational_Unit

Part:BBa_K759009

Designed by: Ami Kawahata   Group: iGEM12_HokkaidoU_Japan   (2012-09-20)
Revision as of 12:56, 20 September 2012 by Registry (Talk | contribs)

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RBS-phaC-RBS-phaA-RBS-phaB

This is a construct of an P3HB (bio plastic) producing operon. RBS(BBa_b0034) is assembled on each phaC, phaA, phaB gene.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 614
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 225
    Illegal NgoMIV site found at 340
    Illegal NgoMIV site found at 574
    Illegal NgoMIV site found at 886
    Illegal NgoMIV site found at 1165
    Illegal NgoMIV site found at 1578
    Illegal NgoMIV site found at 1645
    Illegal AgeI site found at 313
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3583


[edit]
Categories
//function/biosynthesis/plastic
Parameters
None