Reporter

Part:BBa_K863113

Designed by: Moritz Müller   Group: iGEM12_Bielefeld-Germany   (2012-09-20)
Revision as of 12:41, 20 September 2012 by Registry (Talk | contribs)

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Cellulose binding Domain of C. cellulovorans cellulose binding protein with Reporter GFP

Cellulose binding Domain from Clostridium cellulovorans [http://www.ncbi.nlm.nih.gov/nuccore/M73817 cellulose binding protein gene] with T7, RBS linked with two amino acids (GS) to a GFP with His-tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1067
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 978


[edit]
Categories
//binding/cellulose
//function/reporter/fluorescence
//primer/reporter/gfp
//primer/t7
//proteindomain/binding
Parameters
emission511
excitation501
familyCellulose Binding Modul family 3 (pfam00942/cl03026)
functioncellulose binding
originClostridium cellulovorans cellulose binding protein gene (cbp A)
proteinGFPmut3b
strain743B
swissproM73817
tagHis6
typeCellulose binding domain
uniprotP38058