Device
GFP-AID

Part:BBa_K812110

Designed by: Pierre Parutto   Group: iGEM12_Evry   (2012-09-20)
Revision as of 11:01, 20 September 2012 by Registry (Talk | contribs)

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GFP fusion with the ubuquitinase E3 OsTirI recoginition domain for PSC2+ plasmid

This part is a super folded GFP fused to an AID tag for its recignition by the ubiquitinase E3 that induces its degradation in the presence of auxin in the eukaryote cell.

This part is a part of a device pattented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the pattent does not cover the use for oviparian such as frogs and chicken.

The GFP-AID contains 2 NLS domains that relocate the protein in the nucleus of the cells for a better signal concentration.

This part can be used in an oviparian plasmid such as pSC2+ (see K812000)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1020
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1405


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Categories
Parameters
None