Coding

Part:BBa_K883160

Designed by: Hugo de Vries   Group: iGEM12_Wageningen_UR   (2012-09-19)
Revision as of 16:00, 19 September 2012 by Registry (Talk | contribs)

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CCMV_Negative coat protein

This construct produces CCMV core antigen subunits. The subunits have been modified in such a way that they will fold into CCMV Virus-Like Particles that have a negative interior. This was done by replacing 6 Arginine and 3 Lysine residues at the N-Terminus with Glutamic acid residues.

The full protocol for this construct can be found on the Wageningen_UR 2012 Wiki.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 142
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None