![](https://parts.igem.org/images/partbypart/icon_signalling.png)
Signalling
TD1.0
Part:BBa_K750009
Designed by: Sifan Wang,Ruosang Qiu,Xinyi Yao,Qingshu Wu Group: iGEM12_XMU-China (2012-09-19)
TIME DELAY1.0:LuxI(RBS1.0)->LuxR->LuxPR->GFP
Description
This part contains BBa-K750001 and BBa_K750007. Arabinose will activate the promoter Pbad, which will cause production of protein LuxI and LuxR. The protein LuxI, LuxR and LuxPR make up the quorum sensing system, which leads to the expression of GFP.
Performance
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
Illegal NheI site found at 1069 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 793
Illegal BamHI site found at 65
Illegal BamHI site found at 1009 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2024
Illegal BsaI.rc site found at 2751
[edit]
Categories
Parameters
control | K206000 |
device_type | signalling |
proteins | LuxI,LuxR,GFP |
signalling_molecule | AHL |