Signalling
TD1.0

Part:BBa_K750009

Designed by: Sifan Wang,Ruosang Qiu,Xinyi Yao,Qingshu Wu   Group: iGEM12_XMU-China   (2012-09-19)
Revision as of 14:58, 19 September 2012 by AlexYao (Talk | contribs)

TIME DELAY1.0:LuxI(RBS1.0)->LuxR->LuxPR->GFP

Description

This part contains BBa-K750001 and BBa_K750007. Arabinose will activate the promoter Pbad, which will cause production of protein LuxI and LuxR. The protein LuxI, LuxR and LuxPR make up the quorum sensing system, which leads to the expression of GFP.

Performance

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 1069
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 793
    Illegal BamHI site found at 65
    Illegal BamHI site found at 1009
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2024
    Illegal BsaI.rc site found at 2751


[edit]
Categories
Parameters
controlK206000
device_typesignalling
proteinsLuxI,LuxR,GFP
signalling_moleculeAHL