![](https://parts.igem.org/images/partbypart/icon_reporter.png)
Reporter
BM-CFP
Part:BBa_K774004:Design
Designed by: NRP-UEA-Norwich Group: iGEM12_NRP-UEA-Norwich (2012-09-19)
Bacterial-Mammalian promoter with eCFP reporter: BBaK216005 + CArG promoter sequence + BBa_E0420
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 101
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The enhanced Cyan Fluorescence Protein (eCFP) was chosen with the eventual aim of attaching it to part BBa_K774002 or BBa_K774003. It was therefore important that the fluorescence peak of eCFP didn't overlap with RFP too much, as eCFP and RFP will be reporting on either part BBa_K774002 or BBa_K774003 at the same time.
Source
BBaK216005 + BHN + CArG promoter sequence E9-ns2 + BBa_E0420