Signalling
TD0.01
Part:BBa_K750012
Designed by: Sifan Wang,Ruosang Qiu,Jianzhao Chi,Muxin Yu Group: iGEM12_XMU-China (2012-09-19)
TIME DELAY0.01:LuxI(RBS0.01)->LuxR->LuxPR->GFP
This part contains part8 and part10. Arabinose will activate the promoter Pbad, which will cause production of protein LuxI and LuxR. The protein LuxI, LuxR and LuxPR make up the quorum sensing system, which leads to the expression of GFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
Illegal NheI site found at 1068 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 792
Illegal BamHI site found at 65
Illegal BamHI site found at 1008 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2023
Illegal BsaI.rc site found at 2750
[edit]
Categories
Parameters
control | K206000 |
device_type | signaling |
proteins | LuxI,LuxR,GFP |
signalling_molecule | AHL |