![](https://parts.igem.org/images/partbypart/icon_signalling.png)
Signalling
TD0.6
Part:BBa_K750010
Designed by: Sifan Wang, Ruosang Qiu, Shuqin Hu,Zhao Ma,Yunxin Long Group: iGEM12_XMU-China (2012-09-19)
TIME DELAY 0.6:LuxI(RBS0.6)->LuxR->LuxPR->GFP
This part contains part5 and part10. Arabinose will activate the promoter Pbad, which will cause production of protein LuxI and LuxR. The protein LuxI, LuxR and LuxPR make up the quorum sensing system, which leads to the expression of GFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
Illegal NheI site found at 1072 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 796
Illegal BamHI site found at 65
Illegal BamHI site found at 1012 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2027
Illegal BsaI.rc site found at 2754
[edit]
Categories
Parameters
control | K206000 |
device_type | signaling |
proteins | LuxI LuxR GFP |
signalling_molecule | AHL |