Signalling
TD0.6

Part:BBa_K750010

Designed by: Sifan Wang, Ruosang Qiu, Shuqin Hu,Zhao Ma,Yunxin Long   Group: iGEM12_XMU-China   (2012-09-19)
Revision as of 11:44, 19 September 2012 by Registry (Talk | contribs)

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TIME DELAY 0.6:LuxI(RBS0.6)->LuxR->LuxPR->GFP

This part contains part5 and part10. Arabinose will activate the promoter Pbad, which will cause production of protein LuxI and LuxR. The protein LuxI, LuxR and LuxPR make up the quorum sensing system, which leads to the expression of GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 1072
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 796
    Illegal BamHI site found at 65
    Illegal BamHI site found at 1012
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2027
    Illegal BsaI.rc site found at 2754


[edit]
Categories
Parameters
controlK206000
device_typesignaling
proteinsLuxI LuxR GFP
signalling_moleculeAHL