Measurement

Part:BBa_K731702:Design

Designed by: Giacomo Giacomelli, Anna Depetris   Group: iGEM12_TRENTO   (2012-08-24)
Revision as of 11:05, 19 September 2012 by Anna.d (Talk | contribs) (→‎Design Notes)

BBa_K731700 backbone with BBa_K731722 E. coli terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 6850
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6850
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 6856
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6850
    Illegal BglII site found at 6011
    Illegal BamHI site found at 6832
    Illegal XhoI site found at 753
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 6850
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 6850
    Illegal XbaI site found at 6865
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 714
    Illegal NgoMIV site found at 1051
    Illegal NgoMIV site found at 4231
    Illegal NgoMIV site found at 4391
    Illegal NgoMIV site found at 5979
    Illegal AgeI site found at 6800
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2228
    Illegal SapI.rc site found at 3310


Design Notes

The measurements with this plasmid were made using the E. coli strain BL21(DE3)pLysS. This is a lysogen strain that expresses T7 RNA polymerase. The expression in this strain give a very low basal expression.

Source

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References