RNA
Part:BBa_K914000
Designed by: Jean Cury Group: iGEM12_Paris_Bettencourt (2012-09-18)
pLac-supD-T
This part is composed of a tRNA amber suppressor, supD (K228001), which is under the control of pLac (R0011). A terminator is added (B1006, but the sequence is not the exact one, it's not a problem here). This part is used to allow the expression of gene that have one or many amber codon instead of a Serine. Adding IPTG could be usefull when there are to many amber codon in the cell.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 96
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 99
[edit]
Categories
Parameters
//biosafety/semantic_containment
None |