Coding

Part:BBa_K777121:Design

Designed by: Flash Coli   Group: iGEM12_Goettingen   (2012-09-18)
Revision as of 19:09, 18 September 2012 by NikoGoe (Talk | contribs) (→‎Source)

yhjH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • yhjH was amplified from genomic DNA of E. coli DH10B via PCR using the following primers:
    • Fwd: 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATAAGGCAGGTTATCCAGC-3'
    • Rev: 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATAGCGCCAGAACCGCCGTATTCAGC-3'

Source

  • The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).

References

  • Paul K., Nieto V., Carlquist W.C., Blair D.F., Harshey R.M. The c-di-GMP Binding Protein YcgR Controls Flagellar Motor Direction and Speed to Affect Chemotaxis by a “Backstop Brake” Mechanism. Mol Cell. 2010. 38: 128–139.