Protein_Domain
CBDcex
Part:BBa_K863101:Design
Designed by: Moritz Müller Group: iGEM12_Bielefeld-Germany (2012-09-18)
Cellulose binding Domain of Cellulomonas Fimi Exoglucanse (Freiburg-Standard)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 337 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence starts with the Freiburg-prefix (ATG+NgoMIV-restrictionsite) followed by 12 Bases (4 AS) of the exoglucanase gene in front of the CBD. Then 9 bases of the exoglucanase gene behind the CBD. After that the sequence continues with a short GS-Linker.
Source
Cloning-Vector with a CBDcex-Barnase fusion-protein