Protein_Domain
CBDcex

Part:BBa_K863101:Design

Designed by: Moritz Müller   Group: iGEM12_Bielefeld-Germany   (2012-09-18)
Revision as of 18:17, 18 September 2012 by Registry (Talk | contribs)

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Cellulose binding Domain of Cellulomonas Fimi Exoglucanse (Freiburg-Standard)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 337
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence starts with the Freiburg-prefix (ATG+NgoMIV-restrictionsite) followed by 12 Bases (4 AS) of the exoglucanase gene in front of the CBD. Then 9 bases of the exoglucanase gene behind the CBD. After that the sequence continues with a short GS-Linker.


Source

Cloning-Vector with a CBDcex-Barnase fusion-protein

References