Signalling
Part:BBa_K750000:Design
Designed by: Sifan Wang, Yizhen Yan, Jianzhao Chi, Qingshu Wu Group: iGEM12_XMU-China (2012-09-18)
pBADGLT:unstable gfp expression device activated by arabinose
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 800
Design Notes
We use the unstable gfp for our project so the degree of fluorescence can be decreased after it get to a top point. In the cell display part, we can achieve the switch of diffrent numbers.
Source
We built this part by using the biobricks from the dna distribution plates 2012.