Composite

Part:BBa_K917002

Designed by: Elitsa Peeva   Group: iGEM12_Edinburgh   (2012-09-17)
Revision as of 14:12, 17 September 2012 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


lac promoter plus RFP and sacB

The Red Fluorescent Protein (RFP) and SacB, a Bacillus subtilis levansucrase created by Edinburgh team 2010 have been placed under the control of the lac promoter. Function is to test the counterselection efficiency of SacB.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None