Regulatory

Part:BBa_K731201

Designed by: Daniele Rossetto   Group: iGEM12_UNITN-Trento   (2012-08-03)
Revision as of 12:50, 17 September 2012 by Daniele.rossetto (Talk | contribs)

Arabinose inducible araC-pBAD promoter

This E. coli promoter is positively regulated by arabinose.

araC is the regulator protein: in absence of arabinose the protein binds to araI1 and araO2 and the DNA forms a loop that prevents RNA polymerase from binding the promoter. When arabinose is added to the culture it binds to araC, disabling the interaction with araI1 and araO2 sites, and also allowing it to bind araI site, promoting transcription.

Promoter activity is also influenced by glucose levels: in its absence cAMP levels are high and the the complex cAMP+CAP (catabolite activator protein) binds to lacI promoting transcription. In presence of glucose, cAMP levels are low and promoter activity is inhibited.

This part differs in several point mutations from part BBa_I0500, mainly in araC coding sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/positive
//classic/regulatory/other
Parameters
None